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anti human tlr2 antagonist antibodies  (R&D Systems)


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    R&D Systems anti human tlr2 antagonist antibodies
    Anti Human Tlr2 Antagonist Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+tlr2/Human+TLR2+Antibody/pm41198444-227-8-22
    Average 93 stars, based on 68 article reviews
    anti human tlr2 antagonist antibodies - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin
    Article Snippet: Human IL-8 enzyme-linked immunosorbent assay (ELISA) kit was from Becton Dickinson Biosciences (San Jose, CA, USA). .. Recombinant human CD14, human TNF-α, human IL-1α, anti-human MD-2 antibody, and neutralizing antibodies: anti-human CD14, anti-human TLR2 and anti-human TLR4 were purchased from R&D Systems (Minneapolis, MN, USA). .. DL-Sulforaphane and phosphatidylinositol specific phospholipase C (PI-PLC) were purchased from Sigma-Aldrich-Millipore (St. Louis, MO, USA) TAK-242 was from Calbiochem/EMD Millipore (Billerica, MA, USA).

    Incubation:

    Article Title: Activation of Endothelial TLR2 by Bacterial Lipoprotein Upregulates Proteins Specific for the Neutrophil Response
    Article Snippet: .. The top half was incubated with anti-human TLR2 (0.5 μg/ml; AF2616; R&D Systems, Minneapolis, MN) and the bottom half with anti-actin rabbit polyclonal antibody (1:10000; Sigma-Aldrich, St. Louis, MO) overnight at 4°C. .. Binding of the primary antibodies was determined by adding suitable peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA).

    Expressing:

    Article Title: A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
    Article Snippet: Flow cytometry For assessment of CD4 and CCR5 expression, MDMs were cultured in 6-well plates for 16 h in medium alone or with TLR ligands, and immediately after stimulation, gently collected from the plates using cold PBS, washed and stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CCR5 Abs (eBioscience), or with the appropriate isotype control Abs. .. For analysis of TLR expression, unstimulated MDMs were collected as above and stained either under non-permeabilizing conditions (for surface staining) with PEconjugated, anti-human TLR2 or TLR4 (both from eBioscience), or under permeabilizing conditions (for intracellular staining) with PEconjugated, anti-human TLR3 or TLR9 (both from eBioscience), or PE-conjugated anti-human TLR7 (R&D Systems), or with the appropriate isotype control Abs. .. Immunofluorescence intensity was evaluated using a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) with CellQuest software (BD Biosciences), and data were analyzed using FlowJo flow cytometry analysis software (Tree Star, Ashland, OR).

    Staining:

    Article Title: A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
    Article Snippet: Flow cytometry For assessment of CD4 and CCR5 expression, MDMs were cultured in 6-well plates for 16 h in medium alone or with TLR ligands, and immediately after stimulation, gently collected from the plates using cold PBS, washed and stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CCR5 Abs (eBioscience), or with the appropriate isotype control Abs. .. For analysis of TLR expression, unstimulated MDMs were collected as above and stained either under non-permeabilizing conditions (for surface staining) with PEconjugated, anti-human TLR2 or TLR4 (both from eBioscience), or under permeabilizing conditions (for intracellular staining) with PEconjugated, anti-human TLR3 or TLR9 (both from eBioscience), or PE-conjugated anti-human TLR7 (R&D Systems), or with the appropriate isotype control Abs. .. Immunofluorescence intensity was evaluated using a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) with CellQuest software (BD Biosciences), and data were analyzed using FlowJo flow cytometry analysis software (Tree Star, Ashland, OR).

    Control:

    Article Title: A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
    Article Snippet: Flow cytometry For assessment of CD4 and CCR5 expression, MDMs were cultured in 6-well plates for 16 h in medium alone or with TLR ligands, and immediately after stimulation, gently collected from the plates using cold PBS, washed and stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CCR5 Abs (eBioscience), or with the appropriate isotype control Abs. .. For analysis of TLR expression, unstimulated MDMs were collected as above and stained either under non-permeabilizing conditions (for surface staining) with PEconjugated, anti-human TLR2 or TLR4 (both from eBioscience), or under permeabilizing conditions (for intracellular staining) with PEconjugated, anti-human TLR3 or TLR9 (both from eBioscience), or PE-conjugated anti-human TLR7 (R&D Systems), or with the appropriate isotype control Abs. .. Immunofluorescence intensity was evaluated using a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) with CellQuest software (BD Biosciences), and data were analyzed using FlowJo flow cytometry analysis software (Tree Star, Ashland, OR).



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    anti human tlr2 antagonist antibodies  (R&D Systems)
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    <t>TLR2</t> is required for MutuDC sensing of, but not internalization of MRSA (A) Relative pHrodo labeled MRSA internalization by MutuDC over 4 h following stimulation with DapS A8819 (light blue symbols) or DapR A8817 (dark blue symbols), or media alone (white squares). Prior to stimulation, MutuDC were pre-treated for 1 h with TLR2 blocking antibody (clone T2.5; triangles with dashed lines) or media alone (circles with filled lines). Relative MRSA internalization by each DC subset is expressed as the gMFI of pHrodo. Results show the mean and (SD) of duplicates from one experiment, representative of two independent experiments. (B) Cytokine secretion (pg/mL) by MutuDC stimulated with TLR2 ligand peptidoglycan of S. aureus (PGN-SA) (10 μg/mL) or (C) DapS (A8819; light blue) or DapR (A8817; dark blue) MRSA (MOI of 10) for 18 h. MutuDC were first pre-treated with either TLR2 blocking antibody (dot-filled bars) or media alone (filled bars) as in A, or an isotype control (clone 163D3, empty bars) at 1 μg/mL. Results pooled from four (B) or three (C) independent experiments and expressed as the mean ± SEM, with each symbol (circle, square, and directional triangles) representing paired experimental replicates ( n = 3). Statistical significance determined using paired t test and reported as indicated by an ∗ when p ≤ 0.05. (D) Expression of surface activation markers by MutuDC stimulated with DapS A8819 MRSA. DC were pre-treated with TLR2 blocking antibody (black trace), isotype control (dashed red trace), and media alone (light blue shaded). Unstained control sample is shown for each marker (black dashed trace). Data shown from one experiment, representative of three independent experiments.
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    Average 93 stars, based on 1 article reviews
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    Image Search Results


    TLR2 is required for MutuDC sensing of, but not internalization of MRSA (A) Relative pHrodo labeled MRSA internalization by MutuDC over 4 h following stimulation with DapS A8819 (light blue symbols) or DapR A8817 (dark blue symbols), or media alone (white squares). Prior to stimulation, MutuDC were pre-treated for 1 h with TLR2 blocking antibody (clone T2.5; triangles with dashed lines) or media alone (circles with filled lines). Relative MRSA internalization by each DC subset is expressed as the gMFI of pHrodo. Results show the mean and (SD) of duplicates from one experiment, representative of two independent experiments. (B) Cytokine secretion (pg/mL) by MutuDC stimulated with TLR2 ligand peptidoglycan of S. aureus (PGN-SA) (10 μg/mL) or (C) DapS (A8819; light blue) or DapR (A8817; dark blue) MRSA (MOI of 10) for 18 h. MutuDC were first pre-treated with either TLR2 blocking antibody (dot-filled bars) or media alone (filled bars) as in A, or an isotype control (clone 163D3, empty bars) at 1 μg/mL. Results pooled from four (B) or three (C) independent experiments and expressed as the mean ± SEM, with each symbol (circle, square, and directional triangles) representing paired experimental replicates ( n = 3). Statistical significance determined using paired t test and reported as indicated by an ∗ when p ≤ 0.05. (D) Expression of surface activation markers by MutuDC stimulated with DapS A8819 MRSA. DC were pre-treated with TLR2 blocking antibody (black trace), isotype control (dashed red trace), and media alone (light blue shaded). Unstained control sample is shown for each marker (black dashed trace). Data shown from one experiment, representative of three independent experiments.

    Journal: iScience

    Article Title: cGAS/STING sensing in dendritic cells discriminates between daptomycin sensitive and resistant Staphylococcus aureus clinical isolates

    doi: 10.1016/j.isci.2026.115854

    Figure Lengend Snippet: TLR2 is required for MutuDC sensing of, but not internalization of MRSA (A) Relative pHrodo labeled MRSA internalization by MutuDC over 4 h following stimulation with DapS A8819 (light blue symbols) or DapR A8817 (dark blue symbols), or media alone (white squares). Prior to stimulation, MutuDC were pre-treated for 1 h with TLR2 blocking antibody (clone T2.5; triangles with dashed lines) or media alone (circles with filled lines). Relative MRSA internalization by each DC subset is expressed as the gMFI of pHrodo. Results show the mean and (SD) of duplicates from one experiment, representative of two independent experiments. (B) Cytokine secretion (pg/mL) by MutuDC stimulated with TLR2 ligand peptidoglycan of S. aureus (PGN-SA) (10 μg/mL) or (C) DapS (A8819; light blue) or DapR (A8817; dark blue) MRSA (MOI of 10) for 18 h. MutuDC were first pre-treated with either TLR2 blocking antibody (dot-filled bars) or media alone (filled bars) as in A, or an isotype control (clone 163D3, empty bars) at 1 μg/mL. Results pooled from four (B) or three (C) independent experiments and expressed as the mean ± SEM, with each symbol (circle, square, and directional triangles) representing paired experimental replicates ( n = 3). Statistical significance determined using paired t test and reported as indicated by an ∗ when p ≤ 0.05. (D) Expression of surface activation markers by MutuDC stimulated with DapS A8819 MRSA. DC were pre-treated with TLR2 blocking antibody (black trace), isotype control (dashed red trace), and media alone (light blue shaded). Unstained control sample is shown for each marker (black dashed trace). Data shown from one experiment, representative of three independent experiments.

    Article Snippet: mAB mTLR2- anti-mouse/human TLR2 , InvivoGen , Cat# mab-mtlr2; RRID: AB_763722.

    Techniques: Labeling, Blocking Assay, Control, Expressing, Activation Assay, Marker